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40 2019 43권 6호

대한구강악안면병리학회지 제43권 6호 2019

저자 대한구강악안면병리학회지

초록

원 저 1. 허혈성 암세포에서 상피-간엽 이행과 연관된 유전자 발현 프로필 ···················································· 237 박대건 , 김소라 , 우복희 , 이지혜 . 박혜련 2. 비스포스포네이트와 연관된 악골괴사 부골에서의 세균 검출 ······························································ 245 전다니, 최나래, 송재민, 신상훈, 정진 3. RAW 264.7 세포에서 과산화수소에 의한 세포반응을 IP-HPLC로 확인하는 예비연구 ···················· 255 김연숙  증 례 4. 상악에 재발한 거대한 치성 점액섬유종 : 증례 보고 ·········································································· 269 이준상, 신상훈, 전민아

39 2019 43권 6호

허혈성 암세포에서 상피-간엽 이행과 연관된 유전자 발현 프로필

저자 박대건, 김소라, 우복희, 이지혜, 박혜련

초록

Hypoxia is one of the most common features of cancer. It is also associated with cancer progression and the acquisition of aggressiveness, which includes invasion and metastasis. Oral squamous cell carcinoma accounts for 90% of all oral cancers, and its 5-year survival rate is about 50%. Despite various attempts and trials, its prognosis has not improved. Among numerous adverse prognostic factors, hypoxia is suspected as one of the most important factors, as it increases the aggressiveness of oral cancer cells. We attempted to observe the effect of hypoxia on the expression of epithelial-mesenchymal transition markers in oral cancer cells. We analyzed and compared both the mRNA and protein expression levels of epithelial-mesenchymal markers using qRT-PCR and western blotting in both normoxic and hypoxic YD10B oral squamous cell carcinoma cells. Eighty-six genes were analyzed through real-time PCR using commercial microarray plates, performed in triplicate. Among the 86 genes, the expression of 24 were increased (≥ 2 fold) by hypoxia, while that of three genes was decreased (≥ 2 fold). Hypoxia significantly affects epithelial-mesenchymal transition-related genes. Further studies on the regulation of these genes may help to develop more efficient therapeutic modalities for oral cancer and to improve prognosis of oral cancer patients. Key words : Hypoxia, Oral cancer, Epithelial-mesenchymal transition, TCF-4

38 2019 43권 6호

비스포스포네이트와 연관된 악골괴사 부골에서의 세균 검출

저자 전다니, 최나래, 송재민, 신상훈, 정진

초록

The purpose of this study was to identify the oral bacterial species in sequestra from patients with bisphosphonate-related osteonecrosis of the jaw (BRONJ). Fifteen patients with BRONJ (2 males and 13 females) were evaluated. Clinical features, radiographic findings, and bisphosphonate intake history were investigated. All patients were treated with surgical methods (curettage or sequestrectomy). Infected bone samples were collected from the affected BRONJ site. Ten bacterial species were selected for polymerase chain reaction (PCR) detection. Two to nine bacterial species were detected by PCR. Gram-negative species were predominant and all identified bacteria were anaerobes. Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola were detected at high levels. These are major pathogenic species in periodontal disease. Orthopantomographic radiographs showed generalized alveolar bone loss in most patients. These radiographic findings may provide evidence of chronic periodontitis as a pre-existing inflammatory disease. Most patients had experienced a predisposing dental procedure, such as tooth extraction. Sequestra (necrotic bone) infected with oral bacterial species may be an important risk factor for BRONJ. As such, prevention and management of BRONJ may rely on effective control of bacteria in the oral cavity. Key words : BRONJ , Bacteria, Sequestra, Necrotic bone

37 2019 43권 6호

RAW 264.7 세포에서 과산화수소에 의한 세포반응을 IP-HPLC로 확인하는 예비연구

저자 김연숙

초록

Hydrogen peroxide (H2O2) is originally an endogenous small molecule which is reduced into water in cells. In order to know the H2O2-induced oxidative stress in RAW 264.7 cells, first of all, the optimum concentration of exogenous H2O2 which show reactive cellular responses was determined as 40 μM by MTT assay, and followed by 40 μM H2O2 application in RAW 264.7 cells for 30 min, 1, or 2 hours. The expressional changes of essential proteins for cellular proliferation, epigenetic modification, inflammation, apoptosis, survival, and protection were assessed by immunoprecipitation high performance liquid chromatography (IP-HPLC) using 51 antisera. 40 μM H2O2 treatment down-regulated proliferation-related proteins, Ki-67, PCNA, CDK4, cyclin D2, cMyc, and PLK4, induced histone methylation/ deacetylation and DNA methylation by increasing levels of HDAC10 and DMAP1 and by decreasing levels of DNMT1 and KDM4D, activated inflammatory reaction by increasing levels of MCP-1, COX-2, CD68, LTA4H, CXCR4, and lysozyme, and dramatically up-regulated cellular apoptosis-, survival-, and protection-related proteins, AIF, PARP-1, caspase 9, c-caspase 9, pAKT1/2/3, SOD-1, HO-1, NF-kB, NRF2, and GSTO1 in RAW 264.7 cells. These observations suggest exogenous 40 μM H2O2-induced oxidative stresses which resulted global cellular responses including not only antioxidant, inflammation, and apoptosis but also proliferation and epigenetic modification. Particularly, 40 μM H2O2-induced apoptosis was mainly derived from PARP-1/AIF signaling leading parthanatos, and 40 μM H2O2-induced suppression of cMyc/MAX/MAD network was relevant to reduction of RAW 264.7 cell proliferation. Accordingly, H2O2 appears to affect RAW 264.7 macrophages in several ways eliciting not only oxidative stresses but also genome-wide DNA damage. Key words : Hydrogen peroxide, Protein expressions, RAW 264.7 cells, IP-HPLC

36 2019 43권 6호

상악에 재발한 거대한 치성 점액섬유종

저자 이준상, 신상훈, 전민아

초록

The myxoma(odontogenic myxoma, myxofibroma) of the jaws is a benign, usually slow-growing infiltrative tumor of connectice tissue. This article describes a case history of the treatment of recurred odontogenic myxofibroma in the maxilla and further discusses the appropriate management of such cases with reference to the literature review. A 55-year-old male patient presented with a gingival swelling in the right side of maxilla. He had history of second time recurred disease. Microscopically, a high cellularity fibrous tumor with fibroblastic cells was observed. Tumor cells were composed of stellate and spindle shaped fibroblasts. Islands of odontogenic epithelium were seen in the specimen. The final diagnosis was made with odontogenic myxofibroma in consideration of twice-time recurrences and histological characteristics. Because of the high likelihood of recurrence, an accurate differential diagnosis of pathology at the time of initial visit is very important in determining treatment options, and immunohistochemical tests may be helpful. Also close following up is required.(155 words) Key words : Odontogenic Myxoma, Odontogenic Myxofibroma, Recurrence, Surgical management

35 2019 43권 5호

악안면 부위에 발생한 혈관종 및 혈관기형에 대한 후향적 연구

저자 박한결, 금동호, 김윤호, 황대석, 유미현, 김욱규

초록

The vascular origin diseases occurring in the oral and maxillofacial region can be divided into hemangiomas and vascular malformation. They are rare and early lesions are not easy to diagnosis, so they may be misdiagnosed as clinicians, or the method and timing of treatment may be inappropriate. For 10 years, from August 2005 to August 2015, we analyzed the clinical records, diagnosis, treatment, and prognosis of 23 patients with hemangioma or vascular malformation confirmed by clinical, histopathological or radiological examination of maxillofacial lesions at department of oral and maxillofacial surgery, Pusan National University Hospital and Pusan National University Dental Hospital. Chief complants of patients were mainly swelling, mass, and spot-like lesion on various intraoral sites. Treatments were performed with excision, embolization, sclerotherapy, depending on each lesions condition. Arteriovenous malformation cases were more frequently recurred than other lesions. Prognosis of lesions were relatively good but close observation should be needed. Key words : Maxillofacial area, Benign tumor, Vascular malformation, Hemangioma

34 2019 43권 5호

Ninjurin 1이 골모세포의 분화에 미치는 영향

저자 오주환, 최윤정, 유미현, 배문경, 김형준

초록

Nerve injury induced protein 1 (Ninjurin1) was originally described in neuroscience in which the expression of Ninjurin1 was regulated by Schwann cells and dorsal root ganglion neuronal cells of damaged nerve tissues. After the first discovery of Ninjurin1, the widespread expression of Ninjurin1 in adult and embryonic tissues have been observed including bone marrow, peripheral blood lymphocytes, thymus, and heart. Currently, the Ninjurin1 mediated positive regulation of pre-osteoclasts fusion and osteoclast development was reported. The bone homeostasis is dynamically balanced by bone-resorbing activity of osteoclast and bone-forming activity of osteoblast. Until now, the role of Ninjurin1 was never been described in osteoblastogenesis. Therefore, in this study, we have evaluated the expression and function of Ninjurin1 in osteoblast. The ample expression of Ninjurin1 was observed in bone marrow of mouse tibia sections but it was barely expressed in osteocytes. And also the expression levels of Ninjurin1 were gradually increased during osteoblast differentiation of calvarial pre-osteoblast, C2C12, and MC3T3-E1 cells. Importantly, the expression of Ninjurin1 was increased in the absence of osteogenic stimulus, BMP2, which suggests the cell density-dependent regulation of Ninjurin1. The controlled expression of Ninjurin1 by cell-density was evidently shown in not only pre-osteogenic osteoblast lineage cells but also in non-osteogenic cancer cells such as HeLa and A549 cells. In addition, the isoform-specific knockdown of Ninjurin1 remarkably reduced the alkaline phosphatase (ALP)-positive osteoblast differentiation. Thus, our results suggest a previously unappreciated mechanism of Ninjurin1 expression and also suggest its role on osteoblastogenesis. Key words : Ninjurin1, Osteoblast, Cell density, Alkaline phosphatase

33 2019 43권 5호

당뇨환자와 비당뇨환자 간의 두경부 근막간극 감염 시 임상 및 병리학적 특징 비교

저자 최재원, 팽준영, 안재명

초록

This study includes the clinical features of diabetic patients with fascial space infections of head and neck and those of non-diabetic patients. We retrospectively studied 62 patients who were treated for fascial space infections of the head and neck, and compared the patients’ background, the aetiology of the infection, clinical variables, laboratory values, and treatment process. Statistical analyses of the differences between groups were made with Student's t test, the chi square test, and analysis of variance, as appropriate. Diabetic patients had more spaces involved, had longer hospital stays, and developed more complications. The main microorganism of diabetic group was Klebsiella pneumonia and otherwise, Streptococcus spp. was for non-diabetic group. We conclude that diabetic patients are more likely to develop complications and it is necessary to consider about dominant microorganism when using medication for diabetes patients Key words : Facial space infection, Disbetes mellitus, Klebsiella pneumonia

32 2019 43권 5호

저출력 초음파와 기계적 장력이 MG-56 세포의 골모세포 분화에 미치는 영향

저자 최용식, 금동호, 황대석, 유미현, 김규천, 김욱규

초록

Tissue engineering has been rapidly developed in oral and maxillofacial reconstruction. Biocompatible scaffold from chemically composites seeded with stem cells is essential and several growth factors for bone formation and angiogenesis are also required. To overcome limited activity of new bone formation with scaffolds, several biomechanical stimulation methods on cells have been made to grow cells in scaffold. Several bioreactors have been developed for real tissue growth in culture laboratory. In addition to biological stimulants like BMP, growth factors and exogenous drugs, biomechanical stimulation technique has also been known as an effective method in cell differentiation. We developed our own bioreactor with tensile mechanical strains. Then we tested with it for detection of suitable biomechanical effect on the cell differentiation and proliferation. And we also compared the results with the effect of low intensity pulsed ultrasound (LIPUS). Mechanical strain group showed more rapid reaction with cell differentiation and proliferation than non-mechanical strain group. Mechanical strain groups stimulated with 0.5∼0.7Hz for 6 hours and 8 hours showed more active cell differentiation than the group with 0.5∼0.7Hz for 2.5 hours tensile strain stimulation. Group of LIPUS also showed more rapid reaction in cell differentiation and proliferation. LIPUS with 3MHz showed more cell reaction than the LIPUS group with 1MHz. Our results showed the positive effect on differentiation and proliferation of cell with mechanical tensile strain, LIPUS both. Key words : Osteogenic activity, Tensile strain, Low intensity pulsed ultrasound

31 2019 43권 5호

생약 복합 추출물의 인간 골수 유래 중간엽 줄기세포에서의 골 분화에 미치는 영향

저자 김다솔, 성기수, 박민성, 김민경, 박경은, 곽연주, 윤금정, 김형준, 배문경, 배수경, 장혜옥

초록

Herbal medicine has been the basis for medical treatments through much of human history, and such traditional medicine is still widely practiced today. Modern medicine makes use of many plant-derived compounds as the basis for pharmaceutical drugs. In traditionally, Achyranthes aspera, Safflower (Carthamus tinctorius) seed and Acanthopanax senticosus have been used for the treatment and prevention of bone-related diseases. In this study, we investigated the pharmacological effect of mixture of Achyranthes aspera, Safflower (Carthamus tinctorius) seed and Acanthopanax senticosus and the other herbs. Two types of enzymes were used to enhance the extraction components of amino acid, mineral content, free sugar, and flavor recovery in extracting natural herbal mixtures(NME). We evaluated regulation of osteogenic differentiation in human bone marrow mesenchymal stem cells using alkaline phosphatase staining, alizarin red S staining and RT-PCR. The CCK-8 assay indicated that NME had no cytotoxicity but increased cell survival. In addition, NME promoted the mineralization and expression of osteogenic differention marker genes in human bone marrow mesenchymal stem cells. Therefore, NME has an effect of promoting proliferation and osteogenic differentiation of human mesenchymal stem cell. Key words : NME extract, Human bone-marrow mesenchymal stem cells, Osteogenic differentiation, APA

30 2019 43권 5호

점액 선암종에 대한 체계적 고찰과 증계보고 : 림프절 전이가 점액선 암종의 예후를 결정할 수 있는가?

저자 장태환, 박윤하, 조남표, 임대호

초록

Mucinous adenocarcinoma (MAC) refers to a malignant neoplasm that belongs to a subtype of adenocarcinoma which produces mucin. The World Health Organization has classified MAC as a tumor of salivary gland origin and it is extremely rare. Therefore, its characteristics are not well-known. This study reports a case of MAC of minor salivary gland origin, which occurred in the oral commissure of an 82-year-old female patient, and a systematic review on existing cases of MAC of salivary origin. Based on the study’s findings, it is safe to assume that the occurrence of lymph node metastasis is an important factor in determining the prognosis of patients with MAC. Key words : Mucinous adenocarcinoma, Lymphatic metastasis, Prognosis

29 2019 43권 5호

하악골에 발생한 국소 백악-골 형성이상 : 외상성 골낭과 동반한 증례

저자 김기태, 신경수, 최은주, 윤정훈

초록

Benign fibro-osseous lesions are demonstrated as a group of conditions, which normal bone is replaced by a connective tissue matrix that gradually develops cemento-osseous tissue and include developmental lesion, reactive lesion, dysplasia and tumor. Cemento-osseous dysplasia is one of benign fibro-osseous lesions, which is non-neoplastic process. Histologic features of cemento-osseous dysplasia are admixtures of bone and cementoid materials in a fibrous stroma. Cemento-osseous dysplasia associated with traumatic bone cysts has infrequently been reported. Traumatic bone cyst is a pseudocyst that may befound in both the jaws and the long bones. Though blockage of interstitial fluid drainage orvenous obstruction may be the cause of traumatic bone cyst, the exact pathogenesis of traumatic bone cysts is unknown. Melrose et al. was the first to report association of cemento-osseous dysplasia and traumatic bone cystsin their series. It is assumed that different mechanism is involved in cyst formation in these cases. This report presents review of literature and describes a case of focal cemento-osseous dysplasia associated with traumatic bone cyst of the mandible and its surgical treatment. Key words : Traumatic bone cyst, Cemento-osseous dysplasia, Mandible

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