28 2008
가토에서 다양하게 표면처리된 RBM치과용매식체의 생체반응에 대한 실험적 연구
저자
윤종상, 조재오
초록
The purpose of this study was to observe the histopathologic reaction in vital bone to various surface treatedimplants. For this purpose, ten New Zealand Albino rabbits, weighing 3.3 to 4kg were used as experimental animals. Allthe experimental groups divided into five groups; 1) Machined surface as control, 2) RBM(resorbable blast media), 3)RBM etched nitric acid solution, 4) RBM etched sodium hydroxide solution, 5) RBM etched acid, alkali, and heat treatedgroup on each. All the surfaces of implants were examined under the scannning electron microscope to distinguish thedifferences between each experimental groups compare to that on the control group. All the rabbits were implanted intothe tibial metaphyses of rabbits. On the 4th and 8th week after implantations, all the experimental rabbits weresacrificed. All the tissues containing each implanted materials were fixed in ethyl alcohol, and embedded in spurr resinas usual manner, sectioned in 10μm or more thickness, grinded, stained with the Villanuevaʼs osteochrome bone stainmethod and examined histopatholgically. For the fluorescence microscopic examination, three kind of fluorescence dyes,Oxytetracycline, Alizarin-Complexone, and Xylenol-Orange were injected to put into the bone to implant interface producedpolychromatic fluorescence labelling on the 1st week, 2nd week, and 5th week on each. On the 8th week after experiments,the animals sacrificed, and the tissues containing the implants were taken, fixed in ethyl alcohol and embeddedin spurr resin, sectioned, grounded 10um in thickness and examined under the fluorescence microscope. Followingresults were obtained; On the scanning electron microscopic examination of the implants, dull cracks, continuous linearindentations were revealed on the machined surface implant, irregular multiple leaflike eruptions on the RBM, and moresharp porous indentation with multiple complicated c rack s on the RBM acid etched surface, and more dull margins oncomplicated porous indentation on the RBM alkalic etched surface and more dull and less indented particles were notedon the RBM, acid, alkalic etched, heat treated surface, On the histopathologic examination, on the 4th week after experiment,complete osseointegation was noted between the implant and cortical bone on the collar and the apex lesion.and in parts, small newly formed bone spicules directly attached to the screws, and osteoid tissues were revealed inmarrow tissues, in all experimental groups. On the histopathologic examination, on the 8th week after experiment, osseointegrationis more increased compare to that on the 4th week group, the amount of bone trabeculae and osteoid tissuesdirectly fused to screw of implants were markedly increased. On fluorescence examination, band or linear shape waswitnessed on the boarder of compact bone and marrow tissues, and on bone trabeculae according to the formed age. andprecipitated as granular and globular shape on the haversian canals. These results indicate that the surface treatedmethod used for the present study render the implants compatible to bone tissue but the tissue compartibility is not differentamong the surface treated implants.