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년도별 일람

Total 38건 3 페이지
14 2017 41권 3호

ADP 유도 P2Y1단백질에 의한 사람치아 치수세포의 분화 조절

저자 문서윤, 최장열, 안상건

초록

To gain insights into the role of purinergic receptors in human dental pulp cells (hDPCs) differentiation, we characterized the expression and functional activity of P2Y1 receptors and investigated the effects of ADP on the proliferation and differentiation of this pulp stem-like cell population. Our data showed that ADP did not induce cell proliferation to expose the various ADP concentrations for 72 hours, but the proliferative capacity of hDPCs was inhibited at higher ATP concentrations (100 μM). Using RT-PCR analysis, we found that ADP induced several P2Y receptors including P2Y1 as well as odontoblastic differentiation genes, dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) in a dose-dependent manner. The effects of ADP on the expression of DMP-1 and DSPP mRNA were prevented by the P2Y1 antagonist MRS2179. The extracellular matrix calcium deposits were clearly observed in ADP-treated hDPCs by alizarin red S staining. Quantitative measurement of mineralization induced by ADP was significantly inhibited in MRS2179-treated hDPCs. These results may provide new insights into the molecular regulation of the differentiation of hDPCs.

13 2017 41권 2호

좌측 경부 상방에 발생한 비전형 지방종선 종양- 증례보고 및 문헌고찰 -

저자 송두헌, 엄찬용, 임영관, 김병국, 김혜은, 조혜중, SUN QIOACHU, 안규현, SHI SHUHAN, 김옥준

초록

The occurrence of atypical lipomatous tumor of the head and neck is rare, and clinical and histologically differential diagnosis of the tumor is needed for other similar diseases. Herein we report atypical lipomatous tumor arising in neck area. And literature review was done. Complete excision with negative margins followed by long-term follow-up is recommended as the treatment of choice for these uncommon entities.

12 2017 41권 2호

교근에 발생한 고대 신경초종-증례보고-

저자 최은주, 박일경, 정영곤, 최문기, 권경환, 박원종

초록

Schwannoma, benign neoplasia derived from schwann cell, is rare tumor, and prevalent in tongue, palate, floor of mouth and buccal mucosa. Thirty-six-year-old male with asymptomatic protrusion of mandible angle was evaluated. We surgically removed the mass and histological examination gave the diagnosis of ancient schwannoma. We report a case of ancient schwannoma in masseter muscle and review of literature.

11 2017 41권 2호

구강캔디다증 환자의 항진균 치료

저자 김해영, 강재국, 임영관, 김병국, 김혜은, 조혜중, SUN QIOACHU, 안규현, SHI SHUHAN, 김옥준

초록

The aim of this study was to examine the effects and the sensitivity of antifungal therapy for patients with oral candidiasis and to investigate the relationship among the signs & symptoms of patients and the ratio change of Candida species to antifungal therapy. Candida fungus culture test with ChromeIDTM Candida agar (CAN2) was carried out more than twice for 10 patients who visited Oral Medicine department of Chonnam National University Dental Hospital during the period from Dec. 2015 to Aug. 2016. After culturing the smear sample before and after antifungal therapy in ChromeIDTM Candida agar (CAN2), the number of colonies was counted to compare. Patients were divided into 5 group according to the therapeutic effects of the antifungal agents used: 1) high susceptibility to nystatin, 2) low susceptibility to nystatin, 3) high susceptibility to fluzonazole, 4) low susceptibility to fluzonazole, and 5) increased ratio of new Candida species. Although nystatin is used as first-line therapy in oral candidiasis, it is desirable to use fluconazole if patients had a history of the low sensitivity to nystatin or chemotherapy. Even if the patient's symptoms and signs are improved, there is a possibility of oral candidiasis recurring, so that clinicians should be careful during the treatment with antifungal agents.

10 2017 41권 2호

구강질환을 가진 환자들의 캔디다균종의 분포와 채취 방법에 따른 배양 결과 비교

저자 김윤규, 강재국, 임영관, 김병국, 김혜은, 조혜중, SUN QIAOCHU, 안규현, SHI SHUHAN, 김옥준

초록

The purpose of this study is to analyze the distribution of Candida species in patients with oral disease and clarify the distinction of Candida culture test according to its isolation technique. 75 samples was isolated from 42 patients who visited Chonnam National University Dental Hospital due to oral disease from December 2015 to August 2016. For isolating the candida sampling, saliva sampling and oral swabbing were used. Acquired sampling was cultured in CHROMagar Candida Culture Medium, which indicates the candida species as color. Of the 42 patients, C. albicans was the most frequently isolated species in 39 patients. For 17 patients out of 21 who underwent saliva sampling and oral swabbing simultaneously, oral swabbing was quantitatively underestimated comparing to saliva sampling. 12 samples in 21 samples having particular Candida species were not isolated by oral swabbing. Considering the possibility of fungal infection in various oral disease, it is recommended to perform not only oral swabbing but saliva sampling when isolating Candida.

9 2017 41권 2호

투석시킨 커피 추출액에 의한 RAW 264.7 세포의 증식-관련 단백질 발현변화

저자 윤철수, 이석근

초록

Coffee is one of the most familiar beverages to modern human adults, but its bio-physiological effect has not been clearly elucidated. It was known that more than one thousand chemicals were included in the ordinary coffee extract. Among them, the caffein and chlorogenic acid (caffeoylquinic acids) are most abundant and have been investigated by many authors so far. In order to know the real cellular effect of whole coffee extract elements, the dialyzed coffee extract (DCE)1) was made to get coffee elements less than 1000 Da molecular weight, which are freely absorable through gastrointestinal tract. It was directly treated in the culture of RAW 264.7 cells, a murine macrophage lineage. RAW 264.7 cells were treated with DCE equivalent to 2.5 cups of coffee (DCE-2.5), DCE-5, and DCE-10 for 12 hours, and their protein extracts were examined by histological observation and immunoprecipitation high performance liquid chromatography (IP-HPLC). RAW 264.7 cells differently expressed the proliferation-related proteins depending on the dose of DCE. DCE-2.5 and DCE-5 enhanced the cellular growth of RAW 264.7 cells by increasing the expression of β-actin, PCNA, Ki-67, MPM2, MAX, cMyc, E2F-1, and Rb-1, and by decreasing the expression of MAD and p21. These proliferation-related proteins were rarely affected by DCE-10. DCE-2.5 and DCE-5 induced the cellular proliferation of RAW 264.7 cells by the signaling of E2F-1 and cMyc, respectively, but these cellular effects almost disappeared in DCE-10. Therefore, it was presumed that the low dose of coffee, DCE-2.5 and DCE-5 might be effective for the proliferation of murine macrophages, RAW264.7 cells, contrast to the high dose of coffee, DCE-10. It was also suggested that the low dose of DCE-2.5 and DCE-5 be helpful to increase the innate immunity in vivo by increasing the cell number of macrophages in contrast to the high dose of DCE-10.

8 2017 41권 2호

miR-4708의 multidrug resistance protein-1 발현 조절을 통한 paclitaxel 민감성 증가

저자 최장열, 유지원, 안상건

초록

Multidrug resistance (MDR) remains one of the most significant obstacles in various cancer treatment, and this process often involves dysregulation of the number of micro-RNAs. The aim of this study was to explore the role of miR-4708 on the regulation of MDR-1 expression and the regulation of multidrug resistance (MDR) to chemotherapeutic drugs. Luciferase reporter assays demonstrated that miR-4708 directly binds MDR-1 3’-UTR and down-regulated reporter luciferase activity. The mRNA and protein expression levels of MDR1 were significantly decreased following miR-4708 overexpression. Additionally, the accumulation of rhodamine-123 in paclitacel resistant FaDu cells following miR-4708 transfection was significantly increased compared with control, indicating that the efflux capacity was reduced. These results demonstrated that miR-4708 could be involved in the regulation of MDR via targeting MDR-1 and may provide a potential strategy for reversing drug resistance in oral cancer.

7 2017 41권 1호

이하선 하측부에 발생한 모기질종 증례보고

저자 장성백, 신홍인, 김진욱, 최소영

초록

Pilomatricoma is the second most common benign dermal-subcutaneous tumor of the head and neck region, after epidermoid cyst, originating from the outer sheath cells of the hair follicle. It can be easily treated with surgical excision. However, it is a relatively unknown skin lesion to maxillofacial surgeons. Therefore, we report a case of pilomatricoma occuring inferolateral area of the parotid gland in a 54-year-old man; with a review of the relevant literature.

6 2017 41권 1호

법랑모세포섬유-치아종으로부터 발생한 법랑모세포섬유육종-증례 보고-

저자 Bazarsad Shadavlonjid, 조은애, 남웅, 김현실, 육종인, 김진

초록

Ameloblastic fibrosarcoma (AFS) is an extremely rare malignant odontogenic tumor characterized with benign ameloblastic cells islands and malignant mesenchymal component. While two-thirds of AFS seem to arise de novo, but one-third develops from recurrent ameloblastic fibroma (AF) or ameloblastic fibro-odontomas (AFO). Pathological distinction of malignant transformation is essential for appropriate treatment. The patient was a 28 years old man. Since the primary tumor was excised, the mass recurred 2 years later. The recurrent tumor was diagnosed as AFS. Chief complaint was pain in the right mandible. Computer tomography finding revealed multilocular intrabony lesion with radiopaque substance in the primary lesion. In the recurrent lesion cortical bone destruction was found. Microscopically, both the primary and recurrent lesions showed benign ameloblastic follicles with myxoid or highly cellular mesenchymal proliferation. The histological difference between primary and recurrent lesions were that foci of dental hard tissue composed of enamel and dentin were found only in the primary lesion, whereas nuclear pleomorphism was aggrevated in the recurrent lesion. The histological criteria determining malignancy were discussed.

5 2017 41권 1호

악교정수술 전후의 하악하연 피질골 두께 비교연구

저자 윤숙자, 이은주, 김성진, 강병철, 이재서

초록

This study aimed to compare the mandible inferior cortical bone thickness before and after orthognathic surgery on panoramic radiographs. Among 1092 orthognathic surgery patients, 37(12male, 25 female, average age 23.8 yrs± 4.4) were selected who underwent mandible inferior cortex contouring. The total mandible sides which underwent inferior cortex contouring were 62. The mandible inferior cortical bone thickness was measured below the mesial root of the first molar tooth. The mandible inferior cortical bone thickness was 3.49mm± 0.6mm before orthognathis surgery and 1.92 mm± 0.88 mm after surgery, with statistically significant difference between the two(p<.05). The mean contouring amount was 1.57 mm± 0.8 mm. Mandible inferior cortex contouring are often included in orthognathic surgery.

4 2017 41권 1호

비스포스포네이트와 연관된 악골괴사의 임상 및 방사선학 연구

저자 김현기, 강병철, 이재서, 이은주, 윤숙자

초록

The purpose of this study is to evaluate the clinical and radiographic features of Bisphosphonate-related osteonecrosis of the jaws(BRONJ). The clinical and radiographic features of 27 patients diagnosed with BRONJ from 2008 to 2012 were evaluated on the basis of the charts and panoramic radiographs and cone beam computed tomographs. As for clinical features, the following contents were evaluated; type of dental treatments before occurrence of BRONJ, the cause of taking bisphosphonate medicine, undergoing dental treatment, interval between dental treatments and symptom expression. As for radiological features, location of BRONJ, size of bone destructions and sclerosing of the surrounding bone, locational relationship between mandibular canal and inferior border of bone destruction, and effect on the maxillary sinus. In clinical features, extraction was done in 18 patients(66.7%), as the most common dental treatment before occurrence of BRONJ. The most common cause of taking bisphosphonate medicine was osteoporosis(20 pateints, 70.4%). The patients treated during bisphosphonate administration was 17(63.0%). The interval between dental treatments and symptom in the most patients(14 patients, 51.8%) was within 1 month. In radiological features, sequestrum was seen in 11 patients(40.7%) and bony sclerosing in 15(55.6%). The mean width of sclerosing was 32.9±12.4mm and mean height 17.5±4.4mm. Bone destruction invaded to inferior border of mandibular canal in 88.2%(15 among 17 mandibles),. Maxillary sinusitis was diagnosed in 57.1% among the evaluated maxillae(4 among 11 maxillae). In diagnosing BRONJ, clinicians should be cautious about medical history of patients and have well-knowledge of radiographic features.

3 2017 41권 1호

치과용 임플란트를 위한 이종골 이식에서 보이는 우수한 골재건의 조직학적 관찰

저자 김연숙, 이석근

초록

With the multiple practices of bone graft using different artificial bone regenerative substitutes, the bone graft procedures have been widely performed to increase the bony stabilization of dental implant. Xenogenic bone graft materials have been well developed because of their good biocompatibility and abundant source of bone materials. The present study demonstrated the histological findings from excellent bony remodeling in xenogenic bone graft biopsies compared to those findings in autogenous bone graft. For the graft bone biopsies which were usually done in 5-9 months after graft bone insertion, five types of histological grades including excellent, favorable, partial, degenerative, and poor bony remodeling could be assessed to give prognostic information for dental implant. However, recently the xenograft bone materials have been much improved and produced strong osteogenic effect. Among 239 cases of trephine bur-supported core bone biopsy the excellent bony remodeling was found in 20 cases (13.1%) out of 153 xenogenic bone grafts and in 13 cases (43.3%) out of 30 autogenous bone grafts. They produced abundant new bones on the surface of the graft bones in 5–9 months, and the graft bones were partly resorbed and also surrounded by the repetitive deposition of new bone. The osteophytic new bones showed strong birefringence under polarizing microscope, and were gradually elongated and anastomosed with each other to form trabecular bony networks which became proper stress-baring structures for dental implant. Their marrow stromal tissues were composed of loose connective tissue which was well vascularized but rarely infiltrated with inflammatory cells. The present study compared the histological features of excellent bony remodeling between xenogenic and autogenous bone grafts. Although the ratio of excellent bony remodeling in xenogenic bone graft was still low, 13.1%, the recent advance of xenogeic bone products was remarkable in biological aspect and almost comparable to the autogenous bones. Therefore, it was suggested that the xenogenic bone graft will be applicable to the bone regeneration procedures for dental implant with beneficial output in the near future.

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