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Tissue engineering has been rapidly developed in oral and maxillofacial reconstruction. Biocompatible scaffold from chemically
composites seeded with stem cells is essential and several growth factors for bone formation and angiogenesis are also required. To
overcome limited activity of new bone formation with scaffolds, several biomechanical stimulation methods on cells have been made
to grow cells in scaffold. Several bioreactors have been developed for real tissue growth in culture laboratory. In addition to biological
stimulants like BMP, growth factors and exogenous drugs, biomechanical stimulation technique has also been known as an effective
method in cell differentiation. We developed our own bioreactor with tensile mechanical strains. Then we tested with it for detection
of suitable biomechanical effect on the cell differentiation and proliferation. And we also compared the results with the effect of low
intensity pulsed ultrasound (LIPUS). Mechanical strain group showed more rapid reaction with cell differentiation and proliferation than
non-mechanical strain group. Mechanical strain groups stimulated with 0.5∼0.7Hz for 6 hours and 8 hours showed more active cell
differentiation than the group with 0.5∼0.7Hz for 2.5 hours tensile strain stimulation. Group of LIPUS also showed more rapid reaction
in cell differentiation and proliferation. LIPUS with 3MHz showed more cell reaction than the LIPUS group with 1MHz. Our results
showed the positive effect on differentiation and proliferation of cell with mechanical tensile strain, LIPUS both.
Key words : Osteogenic activity, Tensile strain, Low intensity pulsed ultrasound