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Recently, extensive research has been performed in the field of orthopedic medicine to develop cell-based therapies for the restoration
of injured bone tissue. But there has been rarely reported about rehabilitaton of oral and maxillofacial bone defect using self-derived
osteoblasts. Normal human osteoblast cell(NHost) was previously established into marrow-derived human mesenchymal stem cells
for their capacity to proliferate and differentiate into osteoblasts under various culture conditions. The purpose of this study was to
examine proliferation and differentiation of NHosts effected by growth factors with ALP activity and RT-PCR. After NHosts were cultured
under basal and osteogenic medium at 37℃ and 5% CO2, they were analyzed by ALP activity and RT-PCR. BMP-2 under osteogenic
medium decreased growth rate of NHosts compared to under osteogenic medium. BMP-2 under osteogenic medium induced osteoblastic
differentiation in NHosts by increased ALP activity. The differentiating capacity of NHosts under osteogenic medium showed
that NHosts expressed higher mRNA expression levels of OSX and OCN, while that of RUNX2 decreased after BMP-2 treatment. It
suggested that NHosts having characteristics of osteoprecursor cells might be more advanced in their osteogenesis development by
BMP-2, making NHosts an interesting biological tool for treatment of skeletal defects and diseases of oral and maxillofacial bone.