Biology_of_Oral_Mucosa

하악골에_발생한_법랑아세포성_섬유치아종

종합병원_입원환자_가검물에서_분리된_methicillin_resistant_Staphylococcus_aureus의_내성_양상과_분자생물학적_특성

인간_전_타액의_shotgun_proteomic_analysis

사람_골수간엽줄기세포의_증식과_조골세포_분화에_대한_GW9662의_영향

금속산화물_첨가가_다공성_calcium_metaphosphate_ceramic의_생분해성과_조직공학적_골형성능에_미치는_영향

구강_편평세포암종의_침윤성_성장에_미치는_섬유모세포의_영향

한국인에_발생한_전이성_구강종양

The production of insoluble glucan was affected by the concentration of ions and buffers in a mouth. In this study,the effects of ions and buffers on the mRNA expression of gtfB and gtfC gene of Streptococcus mutans which is an importantcausative agent of dental caries were investigated by Fluorescent in situ hybridization(FISH). The mRNA of gtfBand gtfC gene was normally expressed in the BHI broth containing 1 % sucrose. The gtfB and gtfC mRNA expression wasincreased at 0.25 mM and 4 mM of CaCl2, respectively. While the mRNA expression of two genes was inhibited at eachconcentration of KCl and MgCl2 when compared to the control. As for buffers, the gtfB and gtfC mRNA expression waslower than the control by the addition of sodium bicarbonate. The addition of sodium phosphate decreased the gtfBmRNA expression except for 100 mM in which the expression was increased, and the gtfC mRNA expression was similarto or lower than the control at each concentraion of sodium phosphate. The mRNA expression of the gtfB and gtfC wasdecreased at each concentration of potassium phosphate when compared to the control.

The purpose of this study was to observe the histopathologic tissue reaction in vital bone in applying the varioustreated implants. For this purpose, twelve New Zealand Albino rats, weighing 3.3 to 4 kg were used as experimentalanimals. All the experimental groups divided into four groups; Machined surface as control, RBM(resorbable blast media),Hydroxyapatite-sand and Porous coating groups. All the experimental implants were examined under the scannningelectron microscope. All the experimental rabbits were implanted in the tibial metaphyses of rabbits under the generalanesthesia with Ketamine HCl(2.5ML /kg.body wt.) injections. For prevention of infection after implant, prophylactic erythromycineinjections, 250mg/body wt.(Aldrich Co. USA) were performed on each. On the sixth week after implant, allthe experimental rabbits were sacrificed with over dose of Sedaject(Samwoo Pharm .Co. Korea). All the tissues containingeach experimental materials were fixed in ethyle alcohol, and embedded in spurr resin(Polytechnic Co. USA) as usualmanner. sectioned in 12 um thickness, grinded , stained with the Vulenueva's osteochrome stain methed and examinedhistopatholgically. For measuring the distances between the implant and bone without any connective tissue interface,all the distances were calculated the length of the implant direct contact to bones. using the view analyzer program(Korea Optical Co.) and the statistical analysis were performed using the one-way ANOVA test. The statistical differenceswere considered significant below 5% level. Following results were obtained. On the scanning electron microscopicexamination, dull cracked continuous linear indentations were revealed on the machined surface implant, irregularsharp indentation on the resorbabale blast, irregular thin exophytic or indentated leaflets on the hydroxyapatite-sandimplant, and long ovoid globular particles were revealed on the porous coating implant surface respectively. On the histopathologicexamination, complete osseointegation was noted between the implant and cortex bone on the collar and theapex lesion and in parts, small newley formed bone spicules attached to the screws in marrow tissues with compatibilityin all experimental groups, but on the aspect of the tissue compatibility to the various implant materials, the superiorityof the materials could not identified. The ratio of drect contact between the bone and implant, the HA sand gorup wasthe most superior among the gorups and followed by the machine surface, but on RBM and porous coating groups wereinferior compared to that on the experimental groups. With these results, the superiority of tissue compatibility amongthe experimental implant group could not be identified.

Extensive oral mucosa loss from a variety of conditions is associated with significant functional morbidity andmortality. Although it is known that keratinocytes are a rich source of wound healing promoting factors such as transforminggrowth factor-β1(TGF-β1), it is not clear whether differentiated keratinocytes in a multi-layer form releasethis multi-functional growth factor. This study examined the hypothesis that keratinocytes in mono- and multi-layerforms expressed different levels of TGF-β1. When NHOK reached confluency in serum free medium(KBM), in test mediumcontaining 1.2 mM Ca++ KBM NHOK were allowed to form multi-layers and differentiate.The purpose of this study were to investigate the mRNA level of TGF-β1, FGF-2, and TIMP-1 by RT-PCR analysisand also to evaluate the expression of TGF-β1 and involucrin in keratinocytes at different times of the onset ofdifferentiation. The numbers and sizes of these nodules were increased as the process of keratinocyte differentiationproceed. Cultured NHOK in preconfluency under KBM medium expressed a significantly higher level of TGF-β1 relativeto those grown in multi-layer forms, while the level of TGF-β1 mRNA gradually reduced to its lowest level at 7 days ofgrowing cells in test medium. Cultured NHOK in preconfluency of KBM medium expressed a lower level of FGF-2 andTIMP-1 relative to those grown in multi-layer forms, while the level of FGF-2 and TIMP-1 mRNA showed the highestlevel at 3 days at gradually reduced to its lowest level at 7 days of growing cells in test medium. As a differentiationmarker for keratinocytes at different time points, the highest level of involucrin mRNA expression was found at the laterstage of cell differentiation. It suggested that the expression of TGF-β1 mRNA be consistent with the expression ofFGF-2 and TIMP-1 mRNA in NHOK grown in high calcium medium during the terminal differentiation. But differentiatedNHOK expressing higher involucrin mRNA could show constant espression of TGF-β1, FGF-2 and TIMP-1.

Gemcitabine (Gemzar, 2,2-difluorodeoxycytidine, or dFdC) is an analog of cytosine arabinoside with anti-tumor activityin a few human cancers (lung, ovary, pancreatic and breast cancers). However, the mechanism of apoptosis by thiscompound in carcinoma has not been fully elucidated. In the present study, we investigated that gemcitabine alone andcombination with cisplatin or 5-FU are cancer toxicity using lung cancer cell line A549 by MTT, FACS analysis, andWestern blot assay. Also, to confirm enhanced antitumor activity in vivo using an xenograft tumor model. The MTT assayshowed higher cytotoxic effect in combination with gemcitabine-cisplatin or gemcitabine-5-FU than gemcitabinealone. FACS analysis showed that gemcitabine-cisplatin combination increased hypodiploid DNA to 70.84 %. The inductionof apoptosis showed more increase in combination with gemcitabine-cisplatin or gemcitabine-5-FU than gemcitabinealone. The Western results showed higher expression of p53 and p21WAF/CIP1 protein in combination treatment withgemcitabine-cisplatin or gemcitabine-5-FU than gemcitabine treatment alone. But, Bcl-2 protein expression decreased.In vivo experiments showed that more decreased tumor size and more increased survival rate on combination with gemcitabine-cisplatin or gemcitabine-5-FU combination than gemcitabine alone. In conclusion, this study suggests thatgemcitabine combined with cisplatin or 5-FU are the synergistic effect of anticancer therapy on lung cancer.