In this study, we investigated the effect of bisphosphonate on the osteoblastic differentiation of human dental stem cells (hDPSCs). In the first experiment, we evaluated the effect of bisphosphonate on the differentiation of hDPSCs into osteoblasts by alkaline phosphatase staining after culturing hDPSCs. As a result, on day 13, the osteogenic differentiation of hDPSC was suppressed at 5 μM in clodronate and 2 μM in zolendronate. In NBP, osteogenic differentiation is more suppressed. In second experiment, cytotoxicity and proliferation test, the cell proliferation (examined by MTT assay) was more suppressed as the concentrations of zolendronate were larger than those of alendronate and clodronate. Western blotting, a third experiment, was found that AKT phosphorylation was inhibited in cell signaling proteins involved in cell proliferation inhibition and death by bisphosphonate concentration. In human dental stem cells, bisphosphonates inhibit osteoblast differentiation, and this phenomenon is clearly observed in NBPs (zolendronate), and it has been found that it is related to AKT phosphorylation of cell signaling proteins.

Styela clava (SC), a sea squirt has a biphasic life cycle, showed the animal phase during larval life and the vegetable phase during adult life. The adult SC becomes fixed on hard surface and covered with thick cellulose membrane to protect itself from various physical and chemical damages in marine environment. Especially, adult SC forms a huge cystic space as a portion of digestive tract, filled with seawater containing various planktons. In the present study, it is hypothesized that SC can produce secretory materials, like saliva in mammals, to sterilize, to neutralize, and to digest the cystic fluid. First of all, the cyst fluid was examined for the antimicrobial property by bacteria killing assay (BKA) using E. coli, and it resulted that the cyst fluid showed relatively weak bactericidal effect. The cyst fluid was also examined for antioxidant property by DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azinobis-3-ethybenzthiazdine- 6-sulphonic acid) assay, and resulted that the cyst fluid showed strong antioxidant effect comparable to ascorbic acid. The antioxidant materials of cyst fluid were found both in cationic and anionic groups separated by ion exchange column using SP and Q beads, and they were also heat resistant. The strong antioxidant property seems to be originated from the abundant carotenoid materials in the cyst fluid of SC. However, the strong antioxidant materials of the cyst fluid may play a role for the essential antioxidant in the digestive tract of SC. Furthermore, the cyst fluid of SC was examined using SNU-1 cancer cells for its anticancer property. The flow cytometry analysis showed that both of cationic and anionic materials purified from the cyst fluid showed strong anticancer effect on SNU-1 cells. Taken together, the cyst fluid of SC showed weak antimicrobial, but strong antioxidant property and marked anticancer properties in vitro experiment. Therefore, it is presumed that SCs can neutralize the various free radicals of seawater and also protect themselves from carcinogenic impacts in aggressive marine environment by various bioactve substances of their cyst fluid.

Carcinoma ex pleomorphic adenoma (Ca ex PA) is an uncommon malignant tumor. It is an aggressive salivary carcinoma, arising from a primary or recurrent benign pleomorphic adenoma (PA). The most common clinical presentation of Ca ex PA is a firm mass in the parotid gland. Here we report a case of Ca ex PA, arising from a recurrent benign PA in the right parotid gland, in a 70-year old female patient. The lesion was first diagnosed as a PA and a superficial parotidectomy was performed. Re-operation was performed for recurrent PA. The second recurrence was diagnosed as Ca ex PA.

Neurilemmomas, also known as schwannomas, are typically benign, well-encapsulated, slow-growing tumors originating from Schwann cells which encompass peripheral motor and sensory nerves. Neurilemmomas in parapharyngeal space (PPS) are very rare, but they can occasionally originate from the last four cranial nerves and autonomic nerves. In this article, we report the rare case of a 47-year-old male presenting solitary neck swelling and hoarseness. After computed tomography and magnetic resonance imaging, we performed complete surgical excision by a transcervical approach under general anesthesia. In the histologic examination, Neurilemmoma was diagnosed. The patient did not complain of any specific complications, showed good prognosis without recurrence or pain up to date.

Warthin tumor, also known as papillary cystadenoma lymphomatosum is a benign neoplasm which occurs mostly in the parotid gland. It is the second most common benign neoplasm of the parotid gland, following pleomorphic adenoma. In occasion, the size of neoplasm grows slowly, and there is no symptom, except swelling. To prevent the recurrence, some surgeons prefer superficial parotidectomy, but the others prefer local resection with minimal surrounding tissue due to its low recurrence rate. We present a case of Warthin tumor of 55-year-old male.

The purpose of this study is to develop the quantitative PCR(qPCR) assay that would enable the rapid identification and simultaneous detection of six different endodontic pathogenic bacteria in a single reaction. In this study, six pairs of primers for Treponema denticola, Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia, Streptococcus mutans, and Staphylococcus aureus and two pairs of housekeeping genes were designed for a multiplex qPCR based on the SYBR Green method. The genomic DNA was extracted from reference strains and submitted to the qPCR reaction. The specificity of the amplified products was analyzed by melting curves. As a result, six distinct melting peaks were identified by the melting curve analysis and all of the target species were simultaneously discriminated. Therefore, the multiplex qPCR assay developed in this study can be used for rapid identification and detection of T. denticola, P. gingivalis, F. nucleatum, P. intermedia, S. mutans, and S. aureus at the same time. In combination with the melting curve analysis, the level of the target species and total bacterial load can be obtained.

Connective tissue growth factor (CTGF, CCN2) is one of the multi-functional secreted proteins which belong to CCN family of cysteine-rich growth factors. CTGF is known to have pivotal roles in embryonic endochondral ossification but its role in relevance to periodontitis is never been determined. To identify new molecular mediators associated with periodontitis-induced bone resorption, we have analyzed publicly available GEO database and found the markedly augmented CTGF mRNA expression in periodontitis gingival tissues. The existence of CTGF significantly enhanced mature osteoclasts survival which accompanied by reduction in TUNEL-positive nuclei and PARP cleavage. These results may provide another line of evidence the CTGF mediated prolonged osteoclast survival and subsequent increased bone resorption in the periodontitis patients.

This study aimed to compare chin deviation in angle measurement on osteroanterior(PA) cephalogram and computed tomography (CT). Chin deviation was assessed as menton(Me) deviation in angle measurement on PA cephalogram and CT images of 35 patients who had orthognathic surgery (19 male and 16 female with average age of 22.1±3.33yr). The difference between the Me deviation was calculated and statistical analysis was performed to compare the deviation direction and asymmetry severity on the two imaging modalities. There was statistically significant difference in the difference of Me deviation between PA cephalogram and CT by one-simple t-test (p<.05). The mean difference of Me deviation was 2.21°± 1.84°. The maximum difference of Me deviation was 8.64°. There were statistical agreements in the deviation direction (k =0.82, p<.05) and in the severity of facial asymmetry (k =0.37, p<.05). The difference of the deviation direction was detected in 2 patients (5.71 %). The difference of the severity of facial asymmetry was in 15 patients (42.86%). The chin deviation in angle measurement might be different between PA cephalogram and CT in some patients.

Florid cemento-osseous dysplasia (FCOD) is benign and exhibits multifocal involvement of tooth-bearing areas of the jaw. Initially, the lesions are radiolucent but become mixed. In late stages, the lesions change into a radiopaque mass with a thin radiolucent rim. Most FCOD cases are asymptomatic, and conservative treatment is recommended. However, surgical intervention is necessary in secondary infected cases. Because of hypovascularity, infected lesions are difficult to manage and do not respond to antibiotic medications. This clinical report describes the treatment of secondary infection of an FCOD lesion with both conservative and surgical interventions.

This study was performed to analyze the psychologic change of Tae-kwon-do Athletes who were wearing the mouth guard and one of contact sports during their matches of Tae-kwon-do 10 selected Tae-kwon-do athletes who had no injuries which they had college Tae-kwon-do sparring matches in the Cheon-an area have participated in the study. The oral impressions if the selected athletes were taken to produce for individual custom mouth guards. After the mouth guards were produced, each of them sat in a chair and attached G.S.R. surface electrodes in the palms of the ends of the second and third fingers of their left hands without moving their left hands, And then they were asked to watch footages of Tae-kwon-do games to stimulate them as if the had have a mock match, and Labscribe2 which are one of polygraphy was used to record their G.S.R. values. To determine whether the mouth guards affected the changes of their psychological status during the sparring matches of not, the experimental group wore the mouth guards while they watched the footages, and the control group didn’t wear any mouth guards. The G.S.R. values of the two groups were measured while the watched the footages. To see if there were and significant polygraphy differences between the experimental group and the control group in G.S.R. values, a statistical package SPSS was used to make a comparative analysis. And there were significant change in between the experimental and control group that wore the mouth guards, and it was concluded the wearing mouth guards exerts an influence on change in the psychological state of Tae-kwon-do athletes while they watch a footage of mock match.

Coffee (Coffea spp.) is one of the most important agricultural commodities, being widely consumed in the world. Various beneficial health effects of coffee have been extensively investigated, but data on habitual coffee consumption and its bio-physiological effect have not been clearly explained as well as it is not proved the cause and effect between drinking coffee and its bio-physiological reactions. We made the dialyzed coffee extract (DCE), which is absorbable through gastrointestinal tract, in order to elucidate the cellular effect of whole small coffee molecules. RAW 264.7 cells, a murine macrophage lineage, were directly treated with DCE, i.e., DCE-2.5 (equivalent to 2.5 cups of coffee a day), DCE-5, and DCE-10, for 12 hours, and their protein extracts were examined by immunoprecipitation high performance liquid chromatography (IP-HPLC). RAW 264.7 cells differently expressed the inflammation-related proteins depending on the doses of DCE. RAW 264.7 cells treated with DCE showed marked increase of cathepsin C, cathepsin G, CD20, CD28, CD31, CD68, indicating the activation of innate immunity. Particularly, the macrophage biomarkers, cathepsin G, cathepsin C, CD31, and CD68 were markedly increased after DCE-5 and DCE-10 treatments, and the lymphocyte biomarkers, CD20 and CD28 were consistently increased and became marked after DCE-10 treatment. On the other hand, RAW 264.7 cells treated with DCE showed consistent increase of IL-10, an anti-inflammatory factor, but gradual decreases of different pro-inflammatory proteins including TNFα, COX-2, lysozyme, MMP-2, and MMP-3. In particular, the cellular signaling of inflammation was gradually mitigated by the reduction of TNFα, COX-2, IL-12, and M-CSF, and also the matrix inflammatory reaction was reduced by marked deceases of MMP-2, MMP-3, and lysozyme. These anti-inflammatory expressions were consistently found until DCE-10 treatment. Therefore, it is presumed that DCE may have dynamic effects of innate immunity activation and pro-inflammation suppression on RAW264.7 cells simultaneously. These effects were consistently found in the highest dose of coffee, DCE-10 (equivalent to 10 cups of coffee a day in man), that might imply the small coffee molecules were accumulated in RAW 264.7 cells after DCE-10 treatment and produce synergistic cytokine effects for innate immunity activation and anti-inflammatory reaction concurrently.

Although salivary gland adenocarcinoma NOS accounts for third prevalence rate of all salivary gland tumors, it is one of the most aggressive solid tumors. Current therapy does not significantly improve survival rates. Thus, investigating new therapeutic modalities against salivary gland adenocarcinoma NOS is necessary. It is well known that docetaxel(TXT) as an antimicrotubulin agent induces mitotic block in proliferating cells. TXT has significant antitumor effects, and it is currently being tested in patients with malignant tumors, but TXT has not yet been tested in malignant salivary gland tumors. The purpose of this study were to examine the effects of TXT and to evaluate the biological mechanisms of TXT on salivary gland adenocarcinoma NOS. Proliferation, cell cycle regulation, connexin43 expression, apoptosis, and Fas receptor(FasR) expression were measured in cultured SGT cell line. Proliferation was little changed after 10ng/ml TXT exposure, but cellular proliferation was inhibited according to increasing concentration of TXT and time. Especially it was prominently inhibited after 96 hrs at 20ng/ml. G2-M arrest stage showed about up to 5 fold increase after exposure of TXT by flow cytometry. Apoptosis index showed about up to 8 fold increase after exposure of TXT by flow cytometry. Fas expression showed about up to 3 fold increase after exposure of TXT by flow cytometry. Apoptosis showed about up to 3 fold increase at 20ng/ml after exposure of TXT and anti-Fas agonist by flow cytometry. In Immunoslot blotting, Cx 43 protein expression was increased after TXT treatment. It suggested that TXT might induce apoptosis in SGT cells and could be used as a potent and specific chemotherapeutic tool for the treatment of salivary gland adenocarcinoma NOS in future.