The relationship of the down-regulated CXCR-4 expression and metastasis potential in oral cancer cells ; preliminary study
Ji-Soo Hong, Hyun-Kyung Pai, Kyoung-Ok Hong, Jae-Il Lee, Sam-Pyo Hong, Seong-Doo Hong
Dept. of Oral Pathology, College of Dentistry, Seoul National University, 28-2, Yeongeou-dong, Jongno-gu, Seoul, 110-744, Korea.
Chemokine are small molecules that regulate leukocyte trafficking and homing. Their receptors are seven-transmembrane, G-coupled proteins. Recent data suggest that the interactions between chemokines and their receptors are also critical components in the regulation of tumor progression and metastasis in breast cancer and other tumors. Above all, chemokine receptor CXCR-4 and its ligand stromal cell-derived factor-1 (SDF-1) have been shown to mediate organ specific metastasis by creating a chemotatic gradient between the primary tumor site and the metastatic site. But, there are few reports about the expression pattern of chemokine in oral cancer. In this study, we investigated the mRNA expression and protein level of CXCR-4 in several oral cancer cell lines. Specially, KOSCC-25B shows higher CXCR-4 expression, whereas the rest of the cell lines rarely expressed. Also, we induced down-regulation of CXCR-4 expression used by small interfering RNA (siRNA) in KOSCC-25B. In further study, We will investigate the effects on the metastatic potential in oral cancer cells by down-regulation of CXCR-4.