A role of p-AKT on epithelial-mesenchymal transition(EMT) in oral cancer
Kyoung-Ok Hong, Ji-Hong Kim, Ji-Soo Hong, Jae-Il Lee, Sam-Pyo Hong,
Dept. of Oral Pathology, College of Dentistry, Seoul National University, 28-2 Yeongeun-dong, Jongno-gu, Seoul, 110-744, Korea.
Epithelial-mesenchymal transition (EMT) is an important process during development and oncogenesis by which epithelial cells acquire fibroblast-like properties and show reduced intercellular adhesion and increased motility. Also, the process of EMT plays a pivotal role in the conversion of early stage tumors into invasive malignancies, and has been shown to be regulated by the transcriptional factor, Snail. Recently, activation of the phosphatidylinositol 3' kinase (PI3K)/AKT axis is emerging as a central feature of EMT. However, it is unclear whether the phosphorylation of AKT regulate the expression of snail in oral cancer cell underwent EMT. To investigate a role of p-AKT in EMT, we assessed the effects of inhibiting p-AKT activity in oral squamous cancer cells
(KOSCC-25B) using PIAs, structurally modified phosphatidylinositol ether lipid analogues (PIAs). PIAs decreased phosphorylation of a number of downstream targets of AKT, such as extracellular signal regulated kinase (ERK), glycogen synthase kinase 3beta
(GSK-3beta) and NF-kB. These findings suggest that future therapies based on p-AKT inhibition may provide more effective strategy to control tumor cell invasion and metastasis.